Patent · US Expired

Preparation of a water-soluble modified enzyme by covalently bonding an enzyme to a polyurethane pre-polymer/bisulfite adduct

US5134072A · kind A · utility

1Cited by

4References

13Claims

0Family size

Assignee

Boehringer Mannheim GmbH · DE

Inventors

Ulrich Eicken · Korschenbroich, DE
Wilhelm Tischer · Peißenberg, DE

Key dates

Filing date	Sep 13, 1990
Grant date	Jul 28, 1992
Priority date	—
Expiry date	Sep 13, 2010

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12N11/093
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

A water-soluble polyurethane-modified enzyme is prepared by reaction of an enzyme in aqueous solution with an aqueous solution of a water-soluble polyurethane pre-polymer/bisulfite adduct at a pH greater than 7 to covalently bond the enzyme to the adduct. Preferably, a ratio of enzyme to pre-polymer/bisulfite adduct of from 1:10 to 10:1 is used.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.