Mosaic microcolumns, slabs, and separation media for electrophoresis and chromatography
US5135627A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Oct 15, 1990 |
| Grant date | Aug 4, 1992 |
| Priority date | — |
| Expiry date | Oct 15, 2010 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T428/254
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method and compositions for separating molecules based on molecular size, shape, affinity, chirality, weight, charge, and hydrogen bonding, using a mosaic matrix formed by polymerizing a dispersion of dispersoids within a polymeric matrix. The dispersoids and matrix can be of the same or different hydrophobicity or hydrophilicity. The dispersoids can be porous or non-porous. The mosaic matrix can be used with existing chromatographic and electrophoresis apparatus to effect an enhanced separation of molecules, particularly of nucleic acids and peptides, by application of a solution and/or an electrical field to the matrix. The solution can form a pH, ionic, or composition gradient, and be applied using gravity or under pressure. The electrical field can be continuous, pulsed, or two-dimensional.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.