Extraction of granulocyte macrophage colony stimulating factor from bacteria
US5136024A · kind A · utility
0Cited by
4References
13Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Oct 18, 1989 |
| Grant date | Aug 4, 1992 |
| Priority date | — |
| Expiry date | Oct 18, 2009 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S530/825
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method of extracting granulocyte/macrophage colony stimulating factor (GM-CSF) from GM-SCF-expressing bacterial cells comprising treating a suspension of GM-CSF-containing bacterial cells with an acid and an enhancing agent, or with an acid that is itself an enhancing agent, removing substantially all of the suspension liquid from the cells, preparing a second suspension of the acidified cells, neutralizing said second suspension, and separating the GM-CSF-containing liquid from the suspended cells.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.