Method to purify basic fibroblast growth factor
US5136025A · kind A · utility
19Cited by
2References
25Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Apr 4, 1990 |
| Grant date | Aug 4, 1992 |
| Priority date | — |
| Expiry date | Apr 4, 2010 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01D15/3828
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
This invention relates to a method of isolating and purifying proteins such as fibroblast growth factor using metal chelate affinity column chromatography and without the use of heparin. Also disclosed is a method of recovering bFGF multimers and purifying bFGF using metal chelate affinity column chromatography in the absence of heparin.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.