Method for renaturing proteins in the presence of alkyl sulfate detergents
US5136027A · kind A · utility
8Cited by
4References
22Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | May 2, 1989 |
| Grant date | Aug 4, 1992 |
| Priority date | — |
| Expiry date | May 2, 2009 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2740/16122
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A high concentration of renaturing surfactants is added to protein systems in the presence of alkyl sulfate detergents to displace the detergent with respect to the interaction with the protein, thereby renaturing the protein and restoring its reactivity. The presence of higher quantities of detergent in the system results in smaller protein aggregates, and thus a higher reactivity and specificity as measured for the system in its entirety.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.