Method for determining beta-subunit of human prolyl 4-hydroxylase by immunoassay to detect hepatic disease
US5137807A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jul 17, 1989 |
| Grant date | Aug 11, 1992 |
| Priority date | — |
| Expiry date | Jul 17, 2009 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/82
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention is directed to a method for detecting hepatic diseases which are associated with fibrosis by determining the level of human prolyl hydroxylase in a serum sample which comprises: PA0 (a) contacting a serum sample of a patient suspected of having said hepatic disease associated with fibrosis with a monoclonal antibody specific to the .beta.-subunit of human prolyl hydroxylase to form an antigen antibody complex bound on a solid support; PA0 (b) contacting said antigen antibody complex bound on said solid support with an enzyme-labeled monoclonal or enzyme-labeled polyclonal antibody specific to human prolyl hydroxylase to form an antibody antigen enzyme-labeled antibody complex; and PA0 (c) measuring the amount of enzyme activity of said bound antibody antigen enzyme-labeled antibody complex to determine the level of human prolyl hydroxylase present in said serum sample.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.