Rhizobial diagnostic probes and rhizobium trifolii nifH promoters
US5137816A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | May 10, 1991 |
| Grant date | Aug 11, 1992 |
| Priority date | — |
| Expiry date | May 10, 2011 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/878
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
This invention provides useful promoters from the R. trifolii nifH gene for the construction of recombinant molecules to regulate foreign genes for expression under desired conditions. In particular, the promoters act to control expression of the foreign genes within root nodules formed by rhizobial bacterial strains in symbiotic combination with host plants. A rhizobium diagnostic segment (RDS) is also provided comprising a DNA segment found at more than one location in rhizobia, the RDS being species-specific, and detectable by DNA hybridization under stringent conditions. A recombinant plasmid comprising a RDS and a bacterial strain containing the plasmid are provided. Methods are provided for identifying species and strains of field isolates of Rhizobium, using RDS's. One RDS exemplified comprises 5' sequences from the R. trifolii nifH gene.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.