Method for producing the Nde I restriction endonuclease and methylase
US5139942A · kind A · utility
6Cited by
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7Claims
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Inventor
Key dates
| Filing date | May 19, 1988 |
| Grant date | Aug 18, 1992 |
| Priority date | — |
| Expiry date | May 19, 2008 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N9/1007
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention is directed to a method for cloning and producing the Nde I restriction endonuclease by 1) introducing the restriction endonuclease gene from Neisseria denitrificans into a host whereby the restriction gene is expressed; 2) fermenting the host which contains the vector encoding and expressing the Nde I restriction endonuclease, and 3) purifying the Nde I restriction endonuclease from the fermented host which contains the vector encoding and expressing the Nde I restriction endonuclease activity.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.