Method and device employing time-delayed integration for detecting sample components after separation
US5141609A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Nov 16, 1990 |
| Grant date | Aug 25, 1992 |
| Priority date | — |
| Expiry date | Nov 16, 2010 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2030/621
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
This invention relates to a detection method and apparatus useful in capillary electrophoresis and capillary chromatography that employs an array of solid state detector such as a charge-coupled device operating in the time-delayed integration mode which allows more exposure time of the moving analyte zones. The CCD is synchronized so that after a normal exposure of the CCD, the charge information in every row of the CCD is shifted toward one end of the CCD and the charge/signal information in the last row is quantified. Applying the CCD and the time-delayed integration method in effect increases the effective sampling volume of the flow cell without introducing band broadening. Use of the CCD as a fluorescence detection in capillary electrophoresis separations allows analytes to be differentiated both in migration time and fluorescence emission, yielding detection limits for fluorophores in the 1-8.times.10.sup.-20 mole range.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.