Patent · US Expired

Method of producing thrombin from factor II using calcium ions for the conversion on an anion exchanger

US5143838A · kind A · utility

53Cited by

11References

5Claims

0Family size

Assignee

Biotest Pharma GmbH · DE

Inventors

Michael Kraus · Ehrenkirchen, DE
Wolfgang Moller · Lübeck, DE

Key dates

Filing date	Dec 20, 1989
Grant date	Sep 1, 1992
Priority date	—
Expiry date	Dec 20, 2009

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12Y304/21005
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

A method of producing thrombin from Factor II (prothrombin) comprising the steps of: PA1 a) applying a solution of citrated plasma or citrated plasma fraction containing Factor II onto an equilibrated anion exchanger to bind Factor II thereto; PA1 b) applying a solution containing calcium ions to the exchanger to convert the Factor II to thrombin, and PA1 c) selectively eluting the thrombin from the carrier.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.