Construction of an IgG binding protein to facilitate downstream processing using protein engineering
US5143844A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Oct 9, 1990 |
| Grant date | Sep 1, 1992 |
| Priority date | — |
| Expiry date | Oct 9, 2010 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/75
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A recombinant DNA fragment (Z) coding for an immunoglobulin G binding domain related to staphylococcal protein A. The methionine codon of the recombinant DNA fragment has been replaced with a codon of an amino acid residue other than methionine to enable expression of a methionine-free protein. The present invention is also directed to recombinant DNA sequence containing such recombinant DNAS fragment (Z) as well as a recombinant DNA molecule, plasmid vector and bacterial cell containing such recombinant DNAS fragment. Also disclosed is a process for cleaving a fused protein expressed in a biological system using said recombinant DNA fragment.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.