Biologically active reagents prepared from carboxy-containing polymer, analytical element and methods of use

US5147777A · kind A · utility

Assignee

• EASTMAN KODAK COMPANY · US

Inventors

• Richard C. Sutton · Rochester, US
• Susan J. Danielson · Rochester, US
• John B. Findlay · Rochester, US
• Fred T. Oakes · Rochester, US
• Marsha D. B. Oenick · Rochester, US
• Ignazio S. Ponticello · Pittsford, US
• Harold C. Warren, III · Rush, US

Key dates

• Filing date: Jun 18, 1990
• Grant date: Sep 15, 1992
• Expiry date: Jun 18, 2010

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10T428/2998
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Biologically active reagents are prepared from particles of copolymers having highly reactive carboxy or equivalent groups. The reagents are prepared by covalently attaching biologically active substances, for example antibodies, to the particles, directly or indirectly through highly reactive carboxy groups on the particle surface. These reagents are used to advantage in analytical elements, methods for the detection of specific binding ligands (such as immunological species) and immunoassays, and in purification methods as affinity chromatography reagents.