Enzyme electrode and assay for determining LDH5
US5147781A · kind A · utility
Inventors
Key dates
| Filing date | Mar 2, 1987 |
| Grant date | Sep 15, 1992 |
| Priority date | — |
| Expiry date | Mar 2, 2007 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/817
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The invention relates to an assay for the determination of the enzyme lactate dehydrogenase-5 (LDH5) and to a biosensor for such quantitative determination. The assay is based on the interaction of this enzyme with the substrate lactic acid and nicotine-amine adenine dinucleotide (NAD) to yield pyruvic acid and the reduction product of NAD. Anti-LDH5 antibody is bound to a suitable glassy carbon electrode, this is contacted with the substrate containing LDH5, rinsed, inserted into a NAD solution, connected to an amperometric system, lactic acid is added and the current changes are measured, which are indicative of the quantity of LDH-5.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.