Patent · US Expired

Capillary blotting pad for molecular transfer to membranes

US5149408A · kind A · utility

24Cited by
1References
18Claims
0Family size

Assignee

Inventor

Key dates

Filing dateApr 29, 1991
Grant dateSep 22, 1992
Priority date
Expiry dateApr 29, 2011

Classification

  • Technology area (CPC B)Performing Operations; Transporting
  • CPC primaryB01D57/02
  • WIPO fieldChemical engineering
  • WIPO sectorChemistry

Abstract

A capillary blotting sandwich for transferring soluble macromolecules in a liquid medium from a liquid-permeable matrix to a semi-permeable receiving membrane. The blotting sandwich includes: (i) a liquid-permeable matrix layer comprising soluble macromolecules, said matrix having at least one flat surface, (ii) a semi-permeable membrane sheet layer disposed on said flat surface; (iii) an interlocked hydrophilic absorbent fiber-containing material in the form of a non-woven absorbent felt sheet or pad layer disposed on said semi-permeable membrane, wherein said felt layer facilitates capillary transport of a solution through said membrane, whereby said soluble macromolecules in said matrix layer are caused to migrate to said membrane layer.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.