Immunoassay for cell proteins
US5149627A · kind A · utility
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3Claims
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Key dates
| Filing date | Jan 21, 1992 |
| Grant date | Sep 22, 1992 |
| Priority date | — |
| Expiry date | Jan 21, 2012 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/68
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An immunoassay for cellular proteins which may be present as contaminants in a product purified from mammalian cell culture. Since mammalian cell culture requires the use of protein-containing media, the assay must be specific in recognizing cellular proteins but not media proteins. This is accomplished by selective adsorption of an antiserum to cellular proteins against media proteins. Quantification of the assay may be improved through the use of a purified antigen, namely fibronectin, which is known to be highly immunogenic.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.