Chromatographic stationary supports
US5153166A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Aug 18, 1988 |
| Grant date | Oct 6, 1992 |
| Priority date | — |
| Expiry date | Aug 18, 2008 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S530/816
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Methods for modifying polyhydroxylated materials by the direct covalent bonding of nucleophilic ligands to the former sites of hydroxyl groups on the material are disclosed. More specifically, methods for activating the surface of polyhydroxylated materials such as silica, which can serve as stationary phases in various chromatographic methods, are disclosed. The silica is first contacted with a reagent, e.g., a phosphorylating agent, effective to cleave the O--H bond of at least one of said hydroxyl groups and introduce through an --O-- linkage a moiety amenable to nucleophilic displacement; and the product of step (a) is then contacted with a suitable nucleophilic ligand.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.