Recombinant poxvirus host range selection system
US5155020A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Mar 8, 1989 |
| Grant date | Oct 13, 1992 |
| Priority date | — |
| Expiry date | Mar 8, 2009 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2760/20122
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
What is described is a selection system for the cloning and expression of open reading frames in poxviruses, particularly vaccinia virus. The selection system is based on a conditional lethal mutant (host range) of poxviruses. A deletion/recombinant mutant of the vaccinia virus was generated which is capable of plaquing on primary chick embryo fibroblasts and two monkey cell lines (BSC-40 or VERO) but is defective in replication in the human cell line MRC-5. Insertion of the host range gene into the deletion/recombinant restores the ability for growth on MCR-5 cells. A series of plasmids were constructed which allow for the rapid single-step cloning and expression of any open reading frame when recombined with the deletion/recombinant and scored for growth on MCR-5 cells.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.