Insect signal sequences useful to improve the efficiency of processing and secretion of foreign genes in insect systems
US5155037A · kind A · utility
46Cited by
3References
31Claims
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Key dates
| Filing date | Aug 4, 1989 |
| Grant date | Oct 13, 1992 |
| Priority date | — |
| Expiry date | Aug 4, 2009 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2710/14143
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The engineering of foreign vertebrate gene constructs by recombinant DNA techniques for the more efficient processing and secretion of foreign genes in insect systems is done by replacing the foreign vertebrate protein signal peptide sequences with protein signal sequences from insect cell secreted proteins. A baculovirus expression vector system is constructed wherein the natural signal DNA peptide sequence associated with the desired foreign gene, such as CD4 (T cell surface protein T4) is replaced by the signal DNA sequence from the insect signal peptides coding for the cuticle gene or adipokinetic hormone.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.