Blotting technique for membrane assay
US5155049A · kind A · utility
25Cited by
6References
16Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Aug 22, 1989 |
| Grant date | Oct 13, 1992 |
| Priority date | — |
| Expiry date | Aug 22, 2009 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/25375
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A technique for passaging liquid through a membrane putatively containing, in its interstices, at least one substance for which detection is desired, comprises positioning donor and acceptor bibulous matrices onto either surface of the membrane and squeezing the resulting sandwich. This technique permits the application of small volumes of reagents or wash to the membranes and the facile recovery of the waste. The subject membranes are obtained, for example, by blotting developed electrophoresis gels or directly by their use as supports for specific binding assays.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.