Assay device and method using a signal-modulating compound
US5156954A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Mar 8, 1989 |
| Grant date | Oct 20, 1992 |
| Priority date | — |
| Expiry date | Mar 8, 2009 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/525
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An assay device and method for determining the concentration of an analyte. Analyte introduced by liquid absorption into the matrix is used to generate H2O2, via an analyte-specific oxidase, and the H.sub.2 O.sub.2 is utilized by peroxidase in the presence of peroxidase to convert a substrate reagent to a colored reaction product. A trapping compound in the matrix competes with the substrate reagent, in the presence of the peroxidase enzyme, to produce a silent reaction product, thus proportionately reducing the amount of signal reaction product generated by a given amount of analyte. Detection of a wider range of analyte concentrations is therefore possible.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.