Production of biologically active insulin-like growth factor I from high expression host cell systems
US5158875A · kind A · utility
25Cited by
1References
7Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Aug 25, 1989 |
| Grant date | Oct 27, 1992 |
| Priority date | — |
| Expiry date | Aug 25, 2009 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/75
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A novel method for refolding reduced IGF-I is provided in accordance with the present invention. The method is carried out by providing additional positive charge at the amino terminal end of the IGF-I molecule by the presence of a short leader sequence. The additional positive charge on the met-end of the IGF-I molecule has been found to enable recombinant IGF-I to refold simply by stirring solubilized inclusion body protein for between 2-16 hours, or overnight. The present invention also provides a novel fusion protein intermediate of IGF-I attached to the positively charged leader sequence.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.