Patent · US Expired

Method for purifying a crude tissue plasminogen activator preparation

US5158882A · kind A · utility

0Cited by
3References
2Claims
0Family size

Assignee

Inventors

Key dates

Filing dateFeb 28, 1992
Grant dateOct 27, 1992
Priority date
Expiry dateFeb 28, 2012

Classification

  • Technology area (CPC Y)Emerging Cross-Sectional Technologies
  • CPC primaryY10S435/815
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

Separation of tPA and undesired proteins is securely and effectivity accomplished by using a cation-exchanger in the two step procedure for selective elution of undesired proteins from the cation-exchanger on which tPA and undesired proteins are adsorbed: undesired proteins having the pI equivalent to or lower than that of tPA was eluted out in the first step and then undesired proteins having the pI equivalent to or higher than were eluted in the second step.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.