Process for preparing tissue for transplantation
US5160313A · kind A · utility
63Cited by
3References
22Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | May 14, 1991 |
| Grant date | Nov 3, 1992 |
| Priority date | — |
| Expiry date | May 14, 2011 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S623/901
- WIPO fieldBasic materials chemistry
- WIPO sectorChemistry
Abstract
A method is disclosed for preparing a transplantable tissue which has been cryopreserved with an intracellular cryoprotectant and then thawed. After thawing, the tissue is treated with a diluent (or eluent) solution to reduce the level of cryoprotectant in the cells to a substantially non-toxic level. This is conducted using a single dilution step. Cryoprotectants which are removable in this manner include dimethylsulfoxide, glycerol, propanediol and other compounds which penetrate the cells. The dilution step does not require end point titration and can be conducted over a time period greater than about 5 minutes.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.