Test carrier utilizing reaction of two bioaffine binding partners

US5160486A · kind A · utility

Assignee

• Boehringer Mannheim GmbH · DE

Inventors

• Reiner Schlipfenbacher · Lampertheim, DE
• Dieter Mangold · Frankenthal (Pfalz), DE
• Rolf Lerch · Ilvesheim, DE
• Joachim Steinbiss · Lorsch, DE

Key dates

• Filing date: Dec 14, 1989
• Grant date: Nov 3, 1992
• Expiry date: Dec 14, 2009

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S435/805
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Test carrier for analysis of a sample liquid with the help of a specific binding reaction of two bioaffine binding partners, one of which is contained in the sample and one in the reagent system of the test carrier, with several capillary-active test zones (11-16; 21-25) arranged substantially next to one another on a base layer (2). The test zones are in liquid contact with one another so that they form a liquid transport path (20, 30) along which a liquid flows, driven by capillary forces, from a start zone (11, 21), a reaction thereby taking place between the first binding partner and the reagent system containing the second reaction partner, which reaction leads to a labelled species characteristic for the desired analysis. A third binding partner specific for the first binding partner sets as capturing reagent which is so arranged in a zone of the liquid transport path (20, 30) and is so measured with regard to the amount used that it binds a part of the first binding partner and thereby removes it from the reaction leading to the characteristic labelled species. A process for the analysis of a liquid sample with the above test carrier is also provided.