Synthesis of full-length, double-stranded DNA from a single-stranded linear DNA template
US5162209A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Jan 18, 1991 |
| Grant date | Nov 10, 1992 |
| Priority date | — |
| Expiry date | Jan 18, 2011 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6855
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for preparing a ds DNA from a ss DNA template, which method includes: PA0 (a) providing a first DNA strand; PA0 (b) adding a homopolymeric oligonucleotide tail to the 3' end of the first DNA strand, to yield a tailed first DNA strand; PA0 (c) providing a ss homopolymeric oligonucleotide primer complementary to a portion of the tail; PA0 (d) contacting the primer with the tailed first DNA strand; PA0 (e) synthesizing, in the presence of the primer and the tailed first DNA strand, a second DNA strand complementary to the first DNA strand; and PA0 (f) removing the tail and the primer from the first and second DNA strands, respectively; provided that one or both of the tail and the primer contain(s) RNA.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.