Conjugated polypeptides and methods for their preparation
US5162218A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Jun 10, 1991 |
| Grant date | Nov 10, 1992 |
| Priority date | — |
| Expiry date | Jun 10, 2011 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/532
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
Polypeptide compositions are provided having a binding site specific for a particular target ligand and further having an active functionality proximate the binding site. The active functionality may be a reporter molecule, in which case the polypeptide compositions are useful in performing assays for the target ligand. Alternatively, the active functionality may be a chemotherapeutic agent, in which case the polypeptide compositions are useful for therapeutic treatment of various diseased states. A novel method for preparing such polypeptides having active functionalities proximate their binding site comprises combining the polypeptide specific for the target ligand with an affinity label including ligand having a reactive group attached thereto. The reactive group is then covalently attached to an amino acid side chain proximate the binding site and cleaved from the substrate. The substrate is eluted, leaving a moiety of the reactive group covalently attached to the polypeptide. The active funtionality may then be attached to the moiety.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.