Process for recovering purified, oxidized, renatured recombinant interleukin-2 from microorganisms
US5162507A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Sep 12, 1989 |
| Grant date | Nov 10, 1992 |
| Priority date | — |
| Expiry date | Sep 12, 2009 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K14/55
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A process for recovering highly pure, recombinant IL-2 from transformed microorganisms in which the cells are disrupted; impure recombinant IL-2 is isolated in the form of refractile bodies from the disruptate; the impure IL-2 is dissolved and denatured with at least 6M guanidine hydrochloride containing a reducing agent; the reduced IL-2 is precipitated and resolubilized; the reduced solubilized IL-2 therein is oxidized by a controlled oxidation; the oxidized IL-2 is refolded by reducing the concentration of guanidine hydrochloride in the solution; and the oxidized, refolded IL-2 is further purified by ion exchange chromatography or hydrophobic interaction chromatography and ion exchange chromatography.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.