Process and kit for detecting microbial metabolism
US5164301A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jun 22, 1990 |
| Grant date | Nov 17, 1992 |
| Priority date | — |
| Expiry date | Jun 22, 2010 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/80
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A process and kit for detecting microbial metabolism is disclosed, the process including the steps of combining medium, a metabolic dye having an absorbance spectrum which in the presence of microorganisms changes and responds to metabolism of the microorganism and a second analytical dye having an excitation or emission spectrum which overlaps with one of the changed or unchanged absorbance spectra of the first dye. The medium contains a sample solution to be analyzed for containing metabolizing microorganisms. The user observes either no change in the fluorescence emissions of the second analytical dye indicating the absence of metabolizing microorganisms in the sample or a change indicating the presence of metabolizing microorganisms in the sample.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.