Method for producing riboflavin with Candida famata
US5164303A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Feb 15, 1990 |
| Grant date | Nov 17, 1992 |
| Priority date | — |
| Expiry date | Feb 15, 2010 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P25/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Strains of yeast of the species Candida famata are disclosed which can produce 10 grams of riboflavin per liter in 6 days, and in particular, strains identified by ATCC Accession Nos. 20849 and 20850. Riboflavin yields of more than 20 grams per liter in 200 hours have been achieved. Strains of the present invention have increased sensitivity to iron inhibition of flavinogenesis and have enhanced riboflavin production per cell at increased iron concentrations in the fermentation medium. The invention also is directed toward a process for selecting improved microorganisms which are resistant to inhibition of growth by depleted medium. Such selected mircoorganisms are then tested for riboflavin overproduction. The present invention is also directed toward a selection process in which mutated microorganisms are cultured in the presence of tubercidin, a purine analog. Mutant strains resistant to tubercidin are then tested for riboflavin over-production. The present invention also includes a process for producing riboflavin by culturing strains of riboflavin overproducing microorganims in a fermentation medium. Increased riboflavin production can be obtained by elevated iron concentratio…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.