Preparation of an antibody-enzyme conjugate
US5164311A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Mar 19, 1992 |
| Grant date | Nov 17, 1992 |
| Priority date | — |
| Expiry date | Mar 19, 2012 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/972
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An antibody-enzyme conjugate is prepared having an enzyme to antibody ratio of approximately 3. The conjugate is produced by adding sulfhydryl groups to an antibody and maleimidyl groups to an enzyme to produce a modified antibody and enzyme, and reacting the modified antibody and enzyme to produce the conjugate. In producing the modified antibody and enzyme, about a 15 molar excess of reagents for introducing sulfhydryl and maleimidyl groups is used. When reacting the modified antibody and enzyme, a four molar excess of the modified enzyme is used, and reacting is stopped after a specified period of time by addition of selective reagents. The selective reagents may be cysteine and iodoacetamide.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.