Method for lysing hard liposomes using polyethyleneglycol mono-n-alkyl ethers
US5164320A · kind A · utility
3Cited by
2References
23Claims
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Key dates
| Filing date | Mar 5, 1990 |
| Grant date | Nov 17, 1992 |
| Priority date | — |
| Expiry date | Mar 5, 2010 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/829
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for rapidly lysing liposomes having transition temperatures in the range of 35.degree. C. to 65.degree. C. is provided. Such liposomes are treated with a surfactant including polyethyleneglycol mono-n-alkyl ethers such as C.sub.12 H.sub.25 O(CH.sub.2 CH.sub.2 O).sub.n H where n=9-10, a nine mole ethylene oxide adduct of a blend of n-dodecanol, n-tetradecanol and n-hexadecanol, or other appropriate polyethyleneglycol mono-n-alkyl ether capable of causing rapid lysis. The method is applicable to fluorescence immunoassay procedures.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.