In vitro propagation of embryonic stem cells
US5166065A · kind A · utility
121Cited by
0References
16Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | May 31, 1990 |
| Grant date | Nov 24, 1992 |
| Priority date | — |
| Expiry date | May 31, 2010 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2510/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates generally to the use of leukaemia inhibitory factor (LIF) in the maintenance and derivation of embryonic stem (ES) cells in culture. The ES cells are maintained and/or derived from animal embryos by culturing said cells or embryos in a culture medium containing an effective amount of LIF for a time and under conditions sufficient to maintain and/or derive said ES cells. The ES cells may be passaged in LIF and used to make chimaeric animals.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.