Sequencing large nucleic acid fragments
US5167784A · kind A · utility
31Cited by
13References
25Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Sep 4, 1991 |
| Grant date | Dec 1, 1992 |
| Priority date | — |
| Expiry date | Sep 4, 2011 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N27/44773
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The base sequence of large single stranded nucleic acids is determined by retarding the migration rate of a nucleic acid fragment of n bases below a migration rate which would otherwise be the same as the migration rate of a nucleic acid fragment of n bases in a polyacrylamide gel under continuous field gel electrophoresis. A plurality of sequences of electric field pulses is applied to the gel in one dimension.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.