Colorimetric assay for detecting fungal cell envelope perturbations
US5168040A · kind A · utility
1Cited by
3References
13Claims
0Family size
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Key dates
| Filing date | Mar 16, 1988 |
| Grant date | Dec 1, 1992 |
| Priority date | — |
| Expiry date | Mar 16, 2008 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/395
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods of constructing and using gene expression systems to indicate the pertubation of fungal cell envelopes are provided. The recombinant genes produce indicator cleavage enzymes such as .beta.-galactosidase when galactose is able to enter the cell, despite the presence of glucose, through discontinuous cell envelopes. The indicator cleavage enzymes such as .beta.-galactosidase cleaves a colorimetric indicator placed in the media to produce an easily detected measurement of altered cell envelope permeability.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.