Cleavage of targeted RNA by RNAase P
US5168053A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Aug 17, 1990 |
| Grant date | Dec 1, 1992 |
| Priority date | — |
| Expiry date | Aug 17, 2010 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2310/126
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
It has been discovered that it is possible to target any RNA molecule for cleavage by RNase P by forming a hybrid region consisting of a short sequence of base pairs followed by a terminal 3'- NCCA sequence. In the preferred embodiment, the region is formed by addition of an external guide sequence consisting of a nucleotide sequence complementary to the targeted site which includes a 3'-NCCA, wherein the sequence hybridizes to the targeted RNA to form a short sequence of double-stranded RNA under conditions promoting cleavage of the substrate at the nucleotide at the 5' side of the base-paired region by the RNase P or catalytically active equivalent thereof. Specificity is determined by the complementary sequence. The sequence is preferably ten to fifteen nucleotides in length and may contain non-complementary nucleotides to the extent this does not interfere with formation of several base pairs followed by a NCCA at the 3' end. These embodiments are particularly useful in the treatment of viral diseases and disorders associated with expression of specific proteins from mRNA.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.