Protein purification on immobilized metal affinity resins effected by elution using a weak ligand
US5169936A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Apr 14, 1989 |
| Grant date | Dec 8, 1992 |
| Priority date | — |
| Expiry date | Apr 14, 2009 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K14/70514
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
A process for the purification of proteins from solutions containing contaminants of similar net charge and molecular weight is provided, comprising contacting a solution containing the desired protein with an immobilized metal affinity chromatography resin in a buffer containing a low concentration of a weak ligand for the chelant of the resin. The adsorbed protein is then eluted using a buffer having a high concentration of the same weak ligand, e.g., Tris. Particularly preferred features employ agarose-iminodiacetic acid resins having copper cations and are especially useful in obtaining preparations of homogeneous, stable rsT4 proteins.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.