Method of detecting HIV protease activity
US5171662A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Apr 4, 1991 |
| Grant date | Dec 15, 1992 |
| Priority date | — |
| Expiry date | Apr 4, 2011 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/974
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for identifying compounds that inhibit HIV protease is disclosed. A substrate that comprises an HIV protease cleavage site is combined with HIV protease and test compounds. Cleavage of the substrate indicates protease activity and can be detected using antibodies against a cleavage product which do not cross react with uncleaved substrate. A method of detecting the presence of anti-HIV protease antibodies in a sample is also disclosed. A substrate is combined with the sample and HIV protease. Detection of substrate cleavage indicates that the protease is active and that there is an absence of neutralizing anti-HIV protease antibodies.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.