Expression of heterologous DNA using the bacillus coagulans amylase gene

US5171673A · kind A · utility

Assignee

• Biotechnica International, Inc. · US

Inventors

• Alan Sloma · Davis, US
• Nancy M. Hannett · Medford, US
• M. A. Stephens · Concord, US
• Cathy F. Rudolph · Canton, US
• Gerald A. Rufo, Jr. · Burlington, US
• Janice G. Pero · Lexington, US

Key dates

• Filing date: Jul 18, 1988
• Grant date: Dec 15, 1992
• Expiry date: Jul 18, 2008

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/50
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A vector for high-level expression of a heterologous gene in Bacillus, including a DNA sequence which encodes all or part of the structural gene and contains the promoter and ribosome binding site of a Bacillus coagulans amylase gene; within or immediately downstream of the structural gene is a site for insertion of heterologous DNA; when inserted, the heterologous DNA is in the same translational reading frame as the amylase gene; the heterologous DNA thus is under control of the B. coagulans amylase gene regulatory elements and expressed at high levels. A signal-encoding sequence may be inserted independent of, or along with, the heterologous DNA leading to authentic secretion of the heterologous protein.