Cyanocobalamin acid hydrazides and their conjugates

US5171679A · kind A · utility

Assignee

• Boehringer Mannheim GmbH · DE

Inventors

• Erasmus Huber · Garching bei München, DE
• Josef Dieckhoff · Feldafing, DE
• Christian Klein · Iffeldorf, DE
• Konrad Kürzinger · Tutzing, DE

Key dates

• Filing date: Jan 5, 1990
• Grant date: Dec 15, 1992
• Expiry date: Jan 5, 2010

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/82
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The present invention provides cobalamin acid hydrazides of the general formula: EQU B--CO--NH--NH--R--CO--NH--NH).sub.x H (I) wherein B is a residue formed from a cobalamin by the splitting off of a --CONH.sub.2 group, R is a spacer and x is 0 or 1, as well as a process for the preparation thereof. The present invention also provides cobalamin conjugates of the general formula: EQU B--d--CO--NH--NH--R--CO--NH).sub.x N.dbd.GP (II) in which B, R and x have the above-given meanings and d signifies the position of --CO--NH--, as well as a process for the preparation thereof. These cobalamin conjugates are very suitable for use in immunoassays, especially for the determination of cyanocobalamin.