Patent · US Expired

Self-corrected assay device

US5171688A · kind A · utility

30Cited by
31References
14Claims
0Family size

Assignee

Inventors

Key dates

Filing dateAug 23, 1989
Grant dateDec 15, 1992
Priority date
Expiry dateAug 23, 2009

Classification

  • Technology area (CPC Y)Emerging Cross-Sectional Technologies
  • CPC primaryY10T436/104165
  • WIPO fieldMeasurement
  • WIPO sectorInstruments

Abstract

An assay device and method for determining the concentration of an analyte in a fluid sample. The device includes a porous-matrix reservoir which receives the sample to be assayed, and at least two reaction zones into which sample fluid is drawn from the reservoir. In an embodiment containing two reaction zones, a single-point standard curve which corrects for reduced-signal effects related to loss of reagent activity and linear inhibition effects, is used to determine analyte concentration. In an embodiment containing three reaction zones, a two-point standard curve which corrects for reduced-signal effects related loss of reagent activity and non-linear inhibition effects, is used to determine analyte concentration. Also disclosed is an apparatus which uses the device for automated or semi-automated analyte assay.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.