Method of preferential labelling of a phycobiliprotein with a second dye for use in a multiple color assay and product for such use
US5171846A · kind A · utility
25Cited by
6References
10Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | May 21, 1990 |
| Grant date | Dec 15, 1992 |
| Priority date | — |
| Expiry date | May 21, 2010 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/533
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for preparing a phycobiliprotein-Texas Red conjugate which overcomes the energy transfer/fluorescent quenching dilemma is disclosed. A phycobiliprotein, such as phycoerythrin, is conjugated with dye, such as Texas Red, in the presence of a selective salt which causes a hydrophobic intramolecular rearrangement of the phycobiliprotein thereby exposing more hydrophobic sites for binding to Texas Red. The conjugate is useful in multiple color fluorescence assays without requiring the use of multiple exciting sources.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.