Recovery of BT endotoxin protein from lysed cell mixtures
US5173409A · kind A · utility
30Cited by
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25Claims
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Key dates
| Filing date | Dec 8, 1989 |
| Grant date | Dec 22, 1992 |
| Priority date | — |
| Expiry date | Dec 8, 2009 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/832
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Purified endotoxin protein is recovered from aqueous suspensions containing lysed cells and Bacillus thuringiensis crystalline endotoxin protein in a multistep process. The aqueous suspension, e.g., a concentrated fermentation culture, is first treated under strongly basic or acidic conditions to solubilize the crystal protein and the solution is then separated from residual solids. Endotoxin protein is precipitated from the separated aqueous solution, by adjusting its pH to the isoelectric point of the protein, and recovered by centrifugation or the like.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.