Purified chitinases and use thereof
US5173419A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jun 17, 1991 |
| Grant date | Dec 22, 1992 |
| Priority date | — |
| Expiry date | Jun 17, 2011 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S530/823
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Two chitinases from Trichoderma harzianum P1 (ATCC 74058) show chitin-containing-fungus- and insect-inhibiting activity. The first is an endochitinase having a molecular weight of 36 kDa and an isoelectric point of 5.3.+-.0.2. The second is an exochitinase having a molecular weight of 36 kDa an an isoelectric point of 4.4.+-.0.2. The chitinases can be applied in contact with chitin-containing fungus or insect as an inhibitor thereof. The chitinases can be applied to plants or to soil around plants which need production from a chitinase-containing pest. The chitinases can also be used to isolate genes coding for them which can be inserted into a genome of a plant needing protection from a chitinase-containing pest or into the genome of a microorganism to provide transgenic microorganism useful to produce enzyme or as a biocontrol agent.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.