Enhancement of naphthalene dioxygenase activity during microbial indigo production
US5173425A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Aug 4, 1989 |
| Grant date | Dec 22, 1992 |
| Priority date | — |
| Expiry date | Aug 4, 2009 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y114/12012
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
This invention relates to a process for the enhancement of naphthalene dioxygenase activity in organisms that have been transformed with DNA encoding the expression of a multiple component naphthalene dioxygenase enzyme. Cells containing the naphthalene dioxygenase enzyme are capable of producing indigo when cultured in the presence of indole. The invention includes procedures to enhance naphthalene dioxygenase activity by transforming a host cell with a DNA sequence that encodes at least two ferredoxin polypeptides for each reductase polypeptide and iron-sulfur polypeptide, by growing transformed organisms in the presence of iron, or by transforming a host cell with a DNA sequence that encodes a ferredoxin analog.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.