Submicron particles, preparation and utilization in immunodiagnosis
US5175112A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Sep 12, 1990 |
| Grant date | Dec 29, 1992 |
| Priority date | — |
| Expiry date | Sep 12, 2010 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/54393
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention relates to a method of binding an immunological material, selected from antigens and antibodies, to submicron latex particles by covalency or adsorption, in which method, which comprises the stabilization of the latex, PA0 (A) (1) acrylic latex particles based on polybutyl methacrylate, whose mean diameter is less than or equal to 100 nm, said latex containing a molar quantity of butyl methacrylate units greater than 50%, having a density of 0.9 to 1.4 g/cm.sup.3 and containing from 100 to 450 microequivalents of COOH groups per gram of latex, are brought into contact, in an appropriate liquid medium having an ionic strength in the range from 0.01 to 0.5 at a pH of 4 to 10, at a temperature of 0.degree. to 60.degree. C., with PA1 (2) the immunological material to give latex particles sensitized by an immunological material and such that said immunological material is bound to each latex particle, and PA0 (B) said immunological reagent obtained in this way is stabilized by means of a stabilizing material selected from the group consisting of hydroxylated substances containing one or, preferably, several OH groups per molecule, protein-type substances, peptide-t…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.