Process for transforming corn and the products thereof
US5177010A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Sep 5, 1990 |
| Grant date | Jan 5, 1993 |
| Priority date | — |
| Expiry date | Sep 5, 2010 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/8205
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method of producing transformed corn comprising making a wound in a corn seedling in an area of the corn seedling containing rapidly dividing cells and inoculating the wound with vir.sup.+ Agrobacterium tumefaciens. Also, this same method wherein the vir.sup.+ A. tumefaciens contains a vector comprising genetically-engineered T-DNA. There are further provided a transformed corn pollen grain, a corn pollen grain produced by a plant grown from a seedling infected with vir.sup.+ A. tumefaciens, a corn pollen grain produced by a plant grown from a seedling infected with vir.sup..degree. A. tumefaciens containing a vector comprising genetrically-engineered T-DNA, a corn pollen grain whose cells contain a segment of T-DNA, and corn derived from each of these pollen grains. There are also provided a transformed corn plant, a transformed corn plant derived from a corn seedling infected with vir.sup.+ Agrobacterium tumefaciens, a transformed corn plant derived from a corn seedling infected with vir.sup.+ A. tumefaciens containing a vector comprising genetically-engineered T-DNA, and a corn plant whose cells contain a segment of T-DNA. Finally, there are provided transformed corn derived f…
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