Biosensor containing immobilized Zymomonas mobilis cells for measuring glucose, fructose and sucrose
US5177012A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Aug 27, 1990 |
| Grant date | Jan 5, 1993 |
| Priority date | — |
| Expiry date | Aug 27, 2010 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/817
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A biosensor for the determination of glucose and fructose concentrations is provided. The biosensor is produced by a method which comprises treating Zymomonas mobilis whole cells with an organic solvent such as xylene and n-butanol, immobilizing the treated whole cells onto a support selected from the group consisting of gelatin, collagen, agarose, cellophane and polyacrylamide to give an immobilized whole cell enzyme membrane and adhering the membrane to the surface of a pH electrode to give the biosensor. The resulting biosensor is capable of determning glucose and fructose in high concentrations such as 5 g/L and 50 g/L, respectively. Invertase can be immobilized with the whole cells to provide a biosenser for the determination of sucrose.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.