Immunochemical assays for human amylase isoenzymes and related monoclonal antibodies, hydridoma cell lines and production thereof
US5180663A · kind A · utility
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19Claims
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Key dates
| Filing date | Jul 2, 1991 |
| Grant date | Jan 19, 1993 |
| Priority date | — |
| Expiry date | Jul 2, 2011 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K16/40
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Immunochemical assays for human amylase isoenzymes are disclosed. These assays utilize monoclonal antibodies which are produced from hybridoma cell lines and are specific for human salivary amylase and ovarian tumor amylase but not human pancreatic amylase. The assays involve reacting this monoclonal antibody with the unknown sample for the purpose of studying quantitatively and qualitatively the amylase isoenzymes present. The results from such assays are useful in the diagnosis of disease or ruling out disease. Antibody producing hybridoma cell lines and their production are described.
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