Method for purification of mitomycin C
US5180670A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Dec 18, 1990 |
| Grant date | Jan 19, 1993 |
| Priority date | — |
| Expiry date | Dec 18, 2010 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P17/182
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
A method for purification of mitomycin C comprises; PA0 a. a step of treating the culture of a mitomycin C-producing microorganism with reverse phase adsorption resin to adsorb mitomycin C onto the resin; PA0 b. a step of eluting mitomycin C with ethyl acetate out of the resin; PA0 c. a step of adding phosphate buffer to the eluate and then evaporating ethyl acetate; PA0 d. a step of passing, under pressure, the residue remaining after the evaporation through a column packed with a reverse phase adsorbent having a small particle diameter to adsorb mitomycin C onto the resin; PA0 e. a step of eluting mitomycin C with aqueous methanol out of the resin under pressure; PA0 f. a step of treating the eluate with a reverse phase adsorption resin to adsorb mitomycin C onto the resin; PA0 g. a step of eluting mitomycin C with methanol out of the resin; and, PA0 h. a step of concentrating the methanol eluate to crystallize mitomycin C. By this method, mitomycin C can be efficiently purified in an industrial scale.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.