Method for detection and determination of human serum albumin

US5182214A · kind A · utility

Inventors

• Manfred Kessler · Dortmund, DE
• Otto S. Wolfbeis · Regensburg, DE

Key dates

• Filing date: Jul 20, 1990
• Grant date: Jan 26, 1993
• Expiry date: Jul 20, 2010

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/52
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

An optical method for quantitative determination of human serum albumin (HSA) in biological liquids such as urine is based on the observation that the absorption and fluorescence of certain anionic cyanine dyes possessing a terminal cyano group and having the general formula ##STR1## are strongly affected by HSA. This approach is distinctly more sensitive than existing techniques, allowing the detection and quantization of micro-albuminuria (i.e. detection of HSA in the 1-10 mg per liter concentration range) at costs which are distinctly lower than those for existing immunological assays. It is also much more specific for HSA than existing assays based on dye binding. Because the dyes has long wave absorptions and emissions, use can be made of electro-optical components based on semiconductor technology.